FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476794
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCTCGCCACGAGAACACCTCTCAGGTTC47490.9960276345759386No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36990.7758067425345118No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25510.5350319005692186No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG19780.4148542137694685No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG19460.4081427199167775No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT19090.40038255514960336No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17690.37101976954407984No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC16480.3456419334135916No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16100.33767203446352095No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA15880.33305788243979584No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT15810.3315897431595196No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA14980.3141818059791021No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13890.29132078004337303No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC13730.2879650331170275No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT12830.26908895665633376No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC12560.2634261337181257No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA11890.24937394346405367No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT11870.24895447509826044No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10490.2200111578585301No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9950.20868551198211385No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG9530.19987667630045677No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9490.19903773956887041No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA9420.19756960028859422No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA9190.1927457140819725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9130.19148730898459293No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9130.19148730898459293No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8830.185195283497695No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8760.18372714421741884No Hit
CTGCCGAAACTGGCAGCAAACCGTGAGAAAC8560.1795324605594869No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8430.17680591618183117No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG8240.1728209667067958No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8140.17072362487782983No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7750.16254399174486256No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG7740.16233425756196596No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT7500.15730063717244763No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA7480.15688116880665445No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7380.15478382697768847No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7290.1528962193316191No Hit
AAATACAACCTGCAGGTCCGCGGCACCCGTG7160.15016967495396336No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT7070.14828206730789398No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7000.1468139280276178No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA6960.14597499129603142No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6870.14408738364996204No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6610.1386342948946505No Hit
ACCAAAGCCGCTATCAGCCAGCTGGAAAAAC6600.13842456071175394No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6540.13716615561437434No Hit
TACAACCTGCAGGTGCGCGGCACTGCCGGTG6530.13695642143147774No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6440.13506881378540836No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG6440.13506881378540836No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6420.13464934541961518No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC6330.1327617377735458No Hit
AACGACGGTCAGGAAAGCGAGCAGAACACCT6240.13087413012747645No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT6080.12751838320113087No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5820.12206529444581937No Hit
AAACTGGAGGAAGTTGCCGGTAAATACTCCC5810.12185556026292277No Hit
AAATACCACCTGCAGGTTCGTGGCACCCGTG5790.12143609189712957No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5720.1199679526168534No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5680.11912901588526702No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5550.11640247150761125No Hit
AAAGCGGCTCTGCTGGCCCAGGAAGGCGCAA5550.11640247150761125No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG5500.11535380059312828No Hit
AAGTTCAACCTGCAGGTTCGTGGTACCCGCG5480.11493433222733507No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC5460.11451486386154189No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5380.1128369903983691No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5310.11136885111809293No Hit
AACCAGAAAGTCGACGTTCTGACCAAAGCAG5300.11115911693519634No Hit
CTGCAGGTCCGCGGCACCCGTGGTGAACACA5290.11094938275229974No Hit
CGTTACAACCTGCAGGTCCGTGGCACTGCCG5240.10990071183781674No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC5200.10906177510623036No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5120.1073839016430576No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5100.10696443327726439No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4990.10465735726540182No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA4970.10423788889960864No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4940.10360868635091885No Hit
AAAAAGGTTAAAGCTGACCCGGAAGCAGTTA4790.1004626736074699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)