FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518163
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43030.8304336666261389No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT33620.6488305803386193No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27710.5347738066978923No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26920.5195276389861878No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15880.30646726995173335No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC15560.3002916070811694No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14760.2848524499047597No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13770.26574649289895264No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13440.2593778405636836No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13080.25243021983429925No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG12350.23834198891082536No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11090.21402531635798003No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10980.21190243224622368No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG10910.2105515059932878No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10620.2049548115168393No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG10610.20476182205213417No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA10310.1989721381109805No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9970.19241049631100637No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9490.18314700200516054No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC8780.16944475001109688No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8560.16519898178758421No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8430.16269011874641764No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8170.15767239266408448No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8170.15767239266408448No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8110.15651445587585375No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7630.1472509615700079No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7340.14165426709355936No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7320.1412682881641491No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7270.1403033408406235No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7250.13991736191121326No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7070.13644355154652107No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6980.13470664636417498No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6960.13432066743476473No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6920.13354870957594425No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6790.13103984653477768No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6760.13046087814066232No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG6560.12660108884655988No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6250.1206184154407011No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG6230.12023243651129085No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6070.1171446050760089No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5970.11521471042895769No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5820.11231986845838086No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5400.10421431094076573No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5330.10286338468782989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)