FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505035
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27990.5542190145237459No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC26470.5241220905481798No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT26470.5241220905481798No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC18190.3601730573128595No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15000.2970091181799281No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14730.2916629540526894No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT14570.2884948567921035No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG14260.28235666834971834No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12500.24750759848327342No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC11780.23325116081063688No Hit
GGTTGCGGTCGGCGCTGCAGAGGCGCCAGTGCCAGCGGTGCTGG11560.22889502707733128No Hit
CATGCATTTTGGGCGGCCCAGGGCAACGTCCAGATCAGTGCAGT11560.22889502707733128No Hit
CAGAACGTTCAGGGTGCCGGTAGCTGCCGCCGTTTTAATGGTCA10690.21166849822289546No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA10490.20770837664716305No Hit
AACTTCGTGCAGGATAGAGGCCTTAGCGGACGGAATGGTACCCA10330.20454027938657718No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC9940.19681804231389904No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC9380.18572970190184837No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8410.16652311225954636No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT8250.16335501499896046No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8180.16196897244745415No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8170.16177096636866753No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT8040.15919688734444146No Hit
TGGACGCACTTCGTGCAGGATGGACGCTTTTGCGCTCGGGGTAG7780.1540487292959894No Hit
CATGCATTTCGGACGACCCAGTGCCACGTCCAGATCGGTACAGT7280.14414842535665845No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT7220.14296038888393872No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC7030.13919827338699298No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC6850.13563416396883385No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT6660.1318720484718881No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG6590.13048600592038176No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6570.1300899937628085No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6350.1257338600295029No Hit
CAGGTTGCCGTTTACGTAGTGATACAGAGCCACACCTTTTTTCG6170.12216975061134377No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6150.12177373845377053No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT6050.11979367766590435No Hit
GCCGCTGGTAGCCGGTTTGACTTCGTGCAGAATAGACACCTTCG6010.11900165335075788No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5710.1130614709871593No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5670.11226944667201283No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG5590.1106853980417199No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5430.10751730078113399No Hit
ATAACCGCCAGTCATACGGTCCAGTTCGATGAAACCGGACGGGA5400.10692328254477412No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG5390.1067252764659875No Hit
GTATTTCACGGAGCCCTGACCAGACGCAGTACCACCGCCGCGCG5340.10573524607205441No Hit
GTTCAGTACATAGCAGCTGCTAGCCCACAGCAGCTGGGATTTGG5070.1003890819448157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)