FastQCFastQC Report
Sun 21 May 2023
EGAF00007948445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505035
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA58481.1579395487441464No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT32680.6470838654746701No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA28960.5734256041660479No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28440.5631292880691436No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23980.4748185769303118No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG16110.31898779292524276No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15510.3071074281980457No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12320.2439434890651143No Hit
ATGGCTCAGCGTAACGGTATGTCCCCGCGTC12310.2437454829863277No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA11880.23523122159850307No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC10770.2132525468531884No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC10590.20968843743502927No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG10560.2090944191986694No Hit
TCCGTGAAAAGCGGCGAACACGAGCTGATCG10110.20018414565327156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9550.1890958052412209No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA9490.18790776876850118No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8970.17761145267159703No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8710.17246329462314494No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG8700.17226528854435832No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA8640.1710772520716386No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8230.16295900284138723No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG7970.15781084479293517No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA7920.15682081439900203No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC7450.14751452869603096No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA7380.14612848614452462No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG7360.1457324739869514No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7220.14296038888393872No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA7210.14276238280515213No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC6640.13147603631431484No Hit
ATCCCGCTGACCACTACCCCAACCAAATCCC6620.1310800241567416No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6590.13048600592038176No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6350.1257338600295029No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT6350.1257338600295029No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6320.12513984179314305No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG5980.118407635114398No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5880.11642757432653182No Hit
GAAGCGGAAGCGTGGGAACGTCGTGACGGCG5820.11523953785381211No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5780.11444751353866564No Hit
GTGACCGACCTGATGGCACTGGAAGACTCTG5580.11048739196293327No Hit
CCAGTTACGCTGAACAGCGCCGCACCGGCTT5440.10771530685992059No Hit
ATGTCCAACATGGACATCGATGGTATTAACA5400.10692328254477412No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5310.10514122783569456No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC5300.10494322175690794No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5090.10078509410238895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)