FastQCFastQC Report
Sun 21 May 2023
EGAF00007948448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535734
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT57271.0690006607756835No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC49560.9250859568367884No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA36930.6893346324855245No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA29120.5435533305707684No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT27400.5114478453859564No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA26690.49819499975734227No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT25810.48176893756976413No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT24220.4520900297535717No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA22120.41289147226048745No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT18040.33673427484535234No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA15520.28969600585365124No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG15240.28446953152124No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG15240.28446953152124No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT15160.2829762531405511No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG14460.269910067309523No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA10740.20047262260748802No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG10150.18945969454990724No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA10030.18721977697887385No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA9440.176206848921293No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC9020.16836713742267617No Hit
GTGCAGGGACAGATCCAGCGGTGCATCTGGGTCCTCGTAACGTG8920.16650053944681503No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8720.1627673434950927No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG8510.15884748774578428No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC8300.15492763199647588No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8230.15362101341337306No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT7980.14895451847372018No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7830.14615462150992842No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7700.14372804414130894No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7490.13980818839200052No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7450.13906154920165606No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC7260.13551501304751984No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7060.13178181709579753No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6710.1252487241802835No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6130.11442245592028881No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5990.11180921875408319No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5990.11180921875408319No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5500.10266288867236352No Hit
CGGGCCTTCACCTGGATCGTCCGCCGGACCCTGTTCGATAGCAC5480.1022895690771913No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5470.10210290927960518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)