FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535734
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC68541.2793662526552356No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG60181.1233186618732431No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA39050.728906509573781No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT32780.6118708164872866No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG31070.5799519911000609No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC30680.5726722589942024No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG29540.5513930420693851No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA26140.48792871089010587No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG24330.45414328752701905No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT21170.3951587914898065No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG20490.3824659252539506No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC17170.3204948724553603No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA17100.31918825387225747No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC15860.29604243897157917No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT15780.29454916059089026No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC15620.29156260382951243No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC14660.27364326326124533No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11280.21055225167713829No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC10950.20439247835679647No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT9760.18217996244404872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9610.179380065480257No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8500.1586608279481982No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8500.1586608279481982No Hit
TCCTGGGCCCCGAGCGTATGCGCGCTGTCTG8490.15847416815061205No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8240.15380767321095917No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8170.15250105462785638No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8110.15138109584233966No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8000.1493278380688924No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7800.14559464211717008No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7310.13644831203545044No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6970.1301018789175225No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6950.12972855932235028No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6900.12879526033441968No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6650.12412876539476682No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA6480.12095554883580284No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6070.11330249713477211No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6010.11218253834925543No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5870.10956930118304979No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5740.1071427238144303No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5590.10434282685063856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)