FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428066
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA96222.2477842201903444No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA96012.2428784346339117No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC46771.09258852606841No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA39020.9115416781524345No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA33960.7933356071260038No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC28470.6650843561506871No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA28010.6543383496937388No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT26710.623969201011059No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA24050.5618292506295758No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT22280.5204804866539272No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA17640.41208598674036245No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16180.3779790966813529No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG15360.35882317212766257No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT15200.35508543075133275No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14350.3352286796895806No Hit
TTCAGACGGTGGGGTGTCCTCGGACTGGCCCCAGTCGCTCGGGC10970.25626889311461315No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10870.25393280475440705No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA10140.23687935972490226No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9740.22753500628407772No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA9230.2156209556470264No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9120.21305125845079964No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8400.19623142225731546No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG7850.1833829362761817No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC7690.1796451948998519No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7210.16843197077086244No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7080.16539505590259446No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA6570.15348100526554317No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA6280.14670634902094537No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT6260.14623913134890412No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT6070.14180056346451247No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5970.13946447510430635No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC5930.1385300397602239No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG5920.13829643092420327No Hit
GTCAATCAGGGTACAGTTTTTACCATCCAGGATGCGGTGCGGAG5600.13082094817154366No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5510.12871846864735811No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT5480.12801764213929626No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5430.1268495979591932No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5290.12357907425490462No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4960.11586998266622436No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT4960.11586998266622436No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG4920.11493554732214192No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT4920.11493554732214192No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4820.11259945896193578No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG4790.11189863245387394No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG4770.11143141478183272No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG4490.10489036737325554No Hit
CAGTGCATCGATCAGAGTACAGTTTTTGCCATCCAGGATACGAT4450.10395593202917307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)