FastQCFastQC Report
Wed 31 May 2023
EGAF00007948458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420121
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25050.5962567926859167No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22210.5286572201818047No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21580.5136615403657517No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21540.5127094337107643No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19300.4593914610314647No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17730.4220212748232057No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA17180.4089298083171277No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA16870.40155098174097464No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15740.37465396873757795No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA15620.3717976487726155No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15380.3660850088426906No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15340.36513290218770306No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15240.36275263555023435No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC12250.2915826630899193No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12190.2901545031074381No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10710.2549265568729009No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10410.2477857569604947No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9900.23564639710940422No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8850.21065359741598255No Hit
ACGCAGACCGGTCACCATGCGCAGCTTTGCAGAACGTACGTATT8320.19803818423739827No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7870.18732698436878897No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7660.18232842443010466No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7470.17780591781891408No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG7270.1730453845439766No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7180.17090314457025474No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6840.16281023800286107No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA6620.1575736514004299No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6530.15543141142670802No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6480.15424127810797367No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6190.14733850485931435No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5940.14138783826564252No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5770.13734138498194567No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5630.13400901168948945No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5610.13353295836199572No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA5530.1316287450520207No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5470.1302005850695395No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG5430.129248478414552No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT5260.12520202513085515No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT5010.11925135853718333No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT4980.11853727854594272No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG4880.116157011908474No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4680.11139647863353654No Hit
ACCCAGCGGGATGGCCGGCAGAGAATAGCCATCCGGAAAAACGG4670.11115845196978966No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA4600.10949226532356154No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4510.1073500253498397No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC4510.1073500253498397No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4340.10330357206614284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)