FastQCFastQC Report
Wed 31 May 2023
EGAF00007948459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420121
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA47151.122295719566506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39490.9399672951364011No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT38380.9135463354604982No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34520.8216680432542053No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23230.5529359398839858No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21940.5222305002606392No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20000.4760533274937458No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA19720.4693885809088334No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19240.45796330104898353No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17970.42773391475313066No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16910.40250308839596216No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16070.3825088486412248No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15400.3665610621701843No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13060.31086282285341604No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12160.28944042311619744No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC12080.28753620980622246No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9120.2170803173371481No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8350.19875226422863887No Hit
ATCAACTCCAACGCCCCGATGGATAAATGCG8200.1951818642724358No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8050.1916114643162327No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7910.1882790910237765No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7630.18161434443886404No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7560.17994815779263593No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7480.17804394448266095No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7200.17137919789774853No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6820.16233418467536734No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG6650.15828773139167052No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6290.14971877149678306No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6010.14305402491187064No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA5730.13638927832695819No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5640.13424703835323634No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5620.13377098502574258No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5580.1328188783707551No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5570.13258085170700823No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5550.13210479837951447No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA5490.13067663839703322No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG5360.1275822917683239No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5200.12377386514837393No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5110.12163162517465206No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG4980.11853727854594272No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4880.116157011908474No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4700.11187253196103028No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4570.10877818533232093No Hit
ATCCCTCTGGGCATCGACGTAGGTGGCGGTC4550.10830213200482718No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4400.1047317320486241No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4300.10235146541115536No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4270.10163738541991472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)