FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418591
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACAGAAACAGCTGCTGCAGGGTGACG56381.3468994794441354No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55291.3208597413704548No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45571.0886521688235056No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG43251.0332281391620937No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG40000.9555867183002024No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG35190.840677415424603No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25650.6127699831100047No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG22540.538473115762164No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG18060.43144740331254133No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17440.4166358091788882No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13590.3246605875424938No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13490.32227162074674326No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG11190.2673253844444816No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA9780.2336409526243995No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC9540.22790743231459826No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8830.21094576806476967No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8700.207840111230294No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7480.17869471632213785No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7380.17630574952638733No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7010.16746657238211046No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6870.16412201886805974No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6490.15504394504420785No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA6480.1548050483646328No Hit
ACGGCAGCGACTTCCAAAGCCGTTGCTGCCA6470.15456615168505775No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5840.13951566087182954No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5690.1359322106782038No Hit
CGCACCATCTGGGAATCCGCTGCCGATATCG5550.13258765716415308No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5410.12924310365010236No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5170.12350958334030115No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5140.122792893301576No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG4960.11849275306922509No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA4910.11729826967134985No Hit
GCGCTGGCTCGCCTGGACGCCGAACAGCGTG4880.1165815796326247No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG4840.11562599291432447No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4730.11299812943899894No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4570.10917578256579813No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC4550.10869798920664803No Hit
GTTCAGATGCGTAAATCCATGGAAGAACAGA4340.10368115893557195No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4310.10296446889684681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)