FastQCFastQC Report
Wed 31 May 2023
EGAF00007948470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424378
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA33000.7776086413527563No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28300.6668583197055455No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17880.42132250022385703No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16210.381970790191763No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12580.29643383964295983No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT12150.2863013634071512No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA12100.28512316849601066No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT9620.22668470090343984No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA8460.19935057896497935No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC8440.1988793010005231No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7550.1779074315822215No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7280.1715451790620626No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7190.16942442822200962No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6750.15905631300397288No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC6500.15316533844827018No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6380.1503376706615329No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6310.14868819778593612No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5800.1366706096923026No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5730.13502113681670586No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT5720.13478549783447774No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5660.1333716639411091No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5590.13172219106551236No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5550.13077963513659993No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC5540.13054399615437182No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT5290.1246530215986691No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG5180.12206099279415993No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC4900.11546310129177291No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4890.11522746230954478No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4720.11122159961166696No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG4610.10862957080715777No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4530.1067444589493329No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG4510.1062731809848767No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4500.10603754200264857No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4370.10297423523368318No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4310.10156040134031454No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4300.1013247623580864No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4250.10014656744694587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)