FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483528
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39680.8206349994209229No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA39530.8175328005823861No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25650.5304760013897851No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20150.4167287106434374No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19150.39604738505319237No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17720.366473089459142No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16680.3449645108452872No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14480.29946559454674804No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14320.29615658245230886No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13560.28043877500372266No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12320.2547939312718188No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12290.25417349150411145No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11940.24693502754752567No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11300.23369897916976887No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10770.222737876606939No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC10620.21963567776840226No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10420.21549941265035324No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA9620.1989543521781572No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG9470.19585215333962047No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8680.17951390612332688No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC8640.17868665309971707No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7860.16255521913932594No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7710.1594530203007892No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7700.15924620704488673No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7640.15800532750947205No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG7610.15738488774176468No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7500.15510994192683775No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7380.15262818285600835No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7280.15056005029698383No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6970.14414883936400788No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6560.1356694958720074No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6470.13380817656888536No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6300.13029235121854368No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5940.12284707400605549No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5850.12098575470293343No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5800.1199516884234212No Hit
CGTGATCTGGAAGCGGTACGCCAGGCCAAAA5470.11312685097864034No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA5400.11167915818732317No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5330.11023146539600602No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5310.10981783888420113No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5210.10774970632517662No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5140.10630201353385947No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5040.10423388097483496No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5020.10382025446303007No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT4990.1031998146953227No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA4940.10216574841581047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)