FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537408
Sequences flagged as poor quality0
Sequence length44
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC39510.735195605573419No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA36090.6715568060021436No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT35250.655926223651304No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA33200.6177801595808027No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27000.502411575562701No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26800.4986900083363106No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT25450.47356942955817555No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT24520.4562641419554603No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA24460.4551476717875431No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC22890.42593336906037876No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA22150.4121635703227343No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20650.38425181612480647No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT19180.3568982970108372No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG17690.3291726211742289No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17200.32005478146957245No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG17170.3194965463856139No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15620.2906544003810885No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA14080.2619983327378826No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13300.24748422055496008No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC13190.2454373585804454No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12430.23129540312016197No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG12250.2279459926164106No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA11420.21250148862689056No Hit
TACCAGCTCCTGGCCCGGTTTGATCATGTTTTTATCCGCCAGTT11110.2067330594259855No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11030.20524443253542932No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA10550.1963126711920924No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10270.19110247707514588No Hit
TTTAACAATCGCTTTAACCGCTTCTTCACCTTCCACCGCAACAA10050.18700875312611648No Hit
GGTGCCGTTTGCTTTGGCGATGTCGTTTACGGTATCACCCGGTT10010.1862644396808384No Hit
CAGCACGACTTTAGACGCTTTGTTACCGTATTTAACAACATAGG9910.1844036560676432No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG9870.18365934262236513No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9690.1803099321186138No Hit
ACGCACGATTGCTTTGACTGCCTCTTCACCTTCAACGGCAACCA9460.17603012980826485No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA9430.1754718947243063No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9190.17100601405263785No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC8950.1665401333809694No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT8920.16598189829701082No Hit
GAACGCGTTCTGCTGCTCCTTGTTAAATTTGTTATCAGCTTTTG8220.15295641300464452No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8030.14942092413957364No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT7760.14439680838394664No Hit
CCAGAAGGTACGGATCTGGTTTTTGCGTTTATCGTTCAGGGCAC7480.1391866142670001No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC7390.13751190901512444No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT7380.13732583065380494No Hit
TTCATAGAATGCGTTCTGCTGCTCTTTGTTGAACTTGTTATCCG7080.13174347981421936No Hit
TTTGACAATTGCTTTGACAGCCTCTTCACCTTCCACAGCGACTA6820.12690544241991186No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6570.1222534833869239No Hit
ACGATCGCGGCCCTGGAAGTTAACTTTCCACTGAGTCGGGATTT6530.12150916994164583No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT6290.11704328926997738No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA6220.11574074074074073No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG6210.11555466237942122No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA6160.11462427057282362No Hit
TGGAATGGTTTTGCAGATGGATTTGCACAGCTGGTTGTTACCGC6160.11462427057282362No Hit
GAATTTGTTTTGCTGAGCGTCGGCCTTCGGTGCTTGGGAATCAT6070.11294956532094796No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT5950.11071662498511373No Hit
TTTCACGATAGCCTTTACCGCTTCTTCGCCTTCAACAGCGACAA5820.10829760628795998No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5660.1053203525068477No Hit
TTTGGCTTCACCCAGGACGTTGGTGGACTGAGACGGATCGTCCT5600.10420388233893056No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5590.10401780397761104No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG5510.1025291770870549No Hit
CGGTTTATTGCCATCTTCCTTACCCGGTTTGTTGCCATCCTCTT5470.10178486364177683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)