FastQCFastQC Report
Sun 21 May 2023
EGAF00007948565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537408
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC60971.1345197689651065No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA46870.872149279504585No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA37850.704306597594379No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG37120.690722877218054No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA34910.6495995593664404No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA27650.5145066690484696No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27480.5113433369060378No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27310.5081800047636061No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC26240.48826962010241753No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG25850.48101256401095627No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT24510.45607806359414077No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA23710.4411917946885792No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23500.4372841491008693No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT21920.4078837680123854No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA19660.3658300583541741No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG19100.3554096701202811No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC18300.3405234012147196No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17680.32898654281290934No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA14650.27260479933309517No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13790.2566020602596165No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG13780.256415981898297No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13010.24208794807669404No Hit
GAAGATGGCAACAAACCGGGTAAGGAAGACG12770.23762206740502562No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG12370.23017893295224487No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11720.21808383946647614No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG11490.21380403715612717No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG11380.2117571751816125No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11350.21119894009765391No Hit
GTTAAAGTTATCGCGACTGAGGGTGGCGTAC10030.18663659640347743No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9920.1845897344289627No Hit
AACGGTTTTGTAGCTTGGGATTCCCAGTACA9840.18310110753840658No Hit
GTTACCATTATCGCGGGTGAAGGTAACGTCA9560.17789091342146005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9530.1773326783375015No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT9500.1767744432535429No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT9410.17509973800166725No Hit
GCTCAGCACGATGAAGCACAGCAGAACGCGT9410.17509973800166725No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA9010.1676566035488865No Hit
GAACAGCAGAATGCGTTCTACGAAATTCTGC8180.15221209955936643No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8170.15202602119804692No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA7940.14774621888769798No Hit
ACCGACGTTCTGGAATCCGCGACTGCAGACA7470.1390005359056806No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC7450.13862837918304155No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7430.13825622246040253No Hit
CAGAACGCATTCTACGAAATCCTGCACCTGC7410.1378840657377635No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7330.13639543884720734No Hit
GTTAAGGTGGTAAGCGGTGAAGGTAACGTTA6900.12839406931046804No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG6850.12746367750387042No Hit
ATGGAAACCCTGCTGATCGACAACGATGGCT6520.12132309158032631No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG6490.12076485649636776No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC6240.11611289746337977No Hit
GAATGCGACAACTATCGCCAGGAATACGATA6180.11499642729546267No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6170.11481034893414314No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6120.11387995712754556No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6010.11183309515303083No Hit
GTAACCGTCATTGCGCAGCAGGGTGCAGTTC5870.10922799809455759No Hit
GAAGCGCAGAAACTGAACGACTCTCAGGCCC5840.10866976301059901No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5840.10866976301059901No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC5620.1045760390615696No Hit
ACCACCACTTCTACTCAGACGAATAAACCGA5380.10011015838990114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)