FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444280
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA79101.780408751237958No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44330.9977941838480238No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43200.972359773116053No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA39940.8989826235707212No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA36280.8166021427928334No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG32600.7337714954533177No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27310.614702439902764No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC26760.6023228594579995No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23500.5289457099126676No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC21810.49090663545511837No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC21770.4900063023318628No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC17630.3968218240749077No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC17120.3853425767533987No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16430.3698118303772396No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16250.3657603313225894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14410.32434500765283153No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14310.32209417484469255No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12890.29013234896911855No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG12510.28157918429819034No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12350.2779778518051679No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11960.2691996038534258No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC11480.25839560637435854No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10580.2381381111011074No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC10210.22981002971099307No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9910.22305753128657604No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9200.20707661834878907No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT8600.193571621499955No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8510.19154587197262987No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7330.16498604483658955No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG6970.1568830467272891No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6840.1539569640767084No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6820.1535067975150806No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA6800.15305663095345276No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6390.14382821644008284No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5980.1345998019267129No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5860.13189880255694608No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5780.13009813631043485No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC5680.12784730350229584No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5630.12672188709822635No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5560.12514630413252903No Hit
CCGCACCTGCAGTCTGAAACCGGTCCGCCGC5440.12244530476276222No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4990.11231655712613667No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC4910.11051589087962546No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4880.10984064103718376No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4480.10083730980462771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)