FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417072
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60601.4529865346990447No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC45591.093096635592895No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44851.0753538957302335No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA41130.986160662907124No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG25930.6217151954578586No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18990.4553170675566809No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA17830.42750412398818427No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17530.4203111213411593No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17480.4191122875666552No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16200.38842214293934857No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15830.3795507730080178No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG15500.3716384700962903No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12880.3088195803122722No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12580.30162657766524725No Hit
CGTGTTAACAGCAACGTGCTGTACGGTACCG12470.2989891433613381No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11910.2855622050868915No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10520.25223462615567577No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC10490.25151532589097325No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10390.24911765834196492No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG9860.2364100203322208No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9710.23281351900870834No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC9270.2222637817930717No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG7810.18725783557755016No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7450.1786262324011202No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7270.1743104308129052No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7050.1690355622050869No Hit
ATGGCAATTAAACACACCACTATCGACATCG6860.16447999386197107No Hit
CCGTACGATCCGTTCGACCGTTCCCACCTGA6800.16304139333256606No Hit
GAGAAACCGTACGATCCGTTCGACCGCAGCC6150.14745655426401197No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5810.13930448459738368No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5210.1249184793033337No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5190.12443894579353205No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5120.12276057850922624No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5110.1225208117543254No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5110.1225208117543254No Hit
TACATCCTGAAAAAAGGTGAAGAACCGTACG5080.12180151148962289No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4950.11868454367591208No Hit
GTTGTTCCGCCGGGTGCCCCGGCAACTTGCG4730.11340967506809375No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4490.10765527295047378No Hit
ACCGACCTGATTAGCGAAAAATACTACATCC4490.10765527295047378No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4370.1047780718916638No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4320.10357923811715962No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4190.1004622703034488No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA4190.1004622703034488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)