FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425842
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA47061.105104710197679No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA42490.997787911948563No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT40120.9421334673423477No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC36450.8559512683107818No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG33030.7756397912840913No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC32440.7617848873525862No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT28000.6575208645460053No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC19520.4583859741406437No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG18800.4414782947666036No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA16880.39639114976916323No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT15410.3618713043804979No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG14130.3318132077155377No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13390.3144358705811075No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC12370.29048332480121736No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11930.2801508540726373No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA11610.27263632990639725No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG11530.2707576988648372No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG11480.2695835544638622No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9680.22731435602876185No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9290.21815602970115677No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9110.21392910985764674No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8850.20782355897257668No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT8730.20500561241023665No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8700.20430112576965165No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8410.1974910882439966No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8050.18903724855697654No Hit
CACGCAGGCACCACGACGGCGACGAGACGGCGGCGGAGTAGGTT7070.16602401829786634No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC7010.16461504501669633No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC6770.15897915189201628No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT6710.15757017861084627No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA6530.15334325876733623No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG6410.1505253122049962No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6130.14395010355953616No Hit
AGACAGGCTTTTACGAGTGGACGCAACCTGTTTACCGACAGCGT6080.14277595915856114No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG5990.14066249923680613No Hit
TACGCAAGCGCCACGGCGACGACGGCTCGGCGGCGGGGTCGGTT5720.13432211947154107No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG5650.13267831731017607No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5600.13150417290920105No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5560.13056485738842105No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5530.12986037074783605No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5490.12892105522705605No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5160.12117170218062098No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT5000.11741444009750095No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4790.1124830336134059No Hit
GGAAGGCGGCGGGGTCGGTTTCACCGGCACCGGTTTCGGAACTG4700.1103695736916509No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4650.10919542929067588No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA4420.10379436504619084No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG4320.10144607624424083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)