FastQCFastQC Report
Sun 21 May 2023
EGAF00007948595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425842
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT108812.555173045401816No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA81121.9049318761418557No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71491.6787916645140688No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA41280.9693736174449679No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA40970.9620939221589229No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG32540.7641331761545361No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC26640.6255841368394851No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC17360.4076629360185233No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15180.3564702401360129No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14330.3365097853194377No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12740.2991719933684324No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12100.2841429450359523No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11730.27545427646873727No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11420.26817458118269216No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10770.252910703970017No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9790.22989747371090688No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA9780.22966264483071183No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9540.22402675170603178No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9520.22355709394564183No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9150.21486842537842676No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8780.20617975681121165No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC8650.20312698136867666No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8310.19514279944204657No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7890.18527998647385652No Hit
GACAAAGCGGAGGCCCAGTCTACCCCGGAAC7250.17025093814137637No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC7220.16954645150079137No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA7160.16813747821962136No Hit
GACAAAGCGGAAGCTCAGAGCACCCCGGAAC6880.1615622695741613No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA6660.15639603420987128No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6630.15569154756928627No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6170.14488941908031616No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG6100.14324561691895116No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA6060.14230630139817113No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5910.13878386819524613No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5800.1362007505131011No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5580.13103451514881106No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5520.12962554186764105No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5360.125868279784521No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5190.12187618882120599No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5130.12046721554003599No Hit
GCTGAAGCGCAGTCTACCCCGGAGCGTCCGG5030.11811892673808597No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4600.10802128488970088No Hit
GAAGCCGAGCTGGTTAACGACACCACCGCTC4500.10567299608775085No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT4500.10567299608775085No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4420.10379436504619084No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4290.10074158960365581No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT4290.10074158960365581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)