FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403744
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT59361.4702385670127607No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28980.7177815645557581No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA27730.6868213521439328No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC21150.5238467940080843No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20640.5112150273440595No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18970.469852183561861No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18880.46762304826820955No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16960.42006816200364583No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16900.41858207180787826No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14310.3544325116905762No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA14270.3534417848933978No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12790.31678489339779664No Hit
AACTCTCCAACCGCTGAAGAGTGCGGTTACT11150.27616509471348183No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10980.27195450582547354No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10930.27071609732900054No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10540.26105651105651106No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10450.25882737576285963No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9660.23926052151858604No Hit
AACTCTCCAACTGTTGAAGAATGTGGTTATA8670.2147400332884204No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG8440.2090433542046445No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8170.20235594832369025No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7920.1961639058413252No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7710.19096259015613856No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7660.18972418165966554No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7490.18551359277165727No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7460.1847705476737735No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7260.17981691368788144No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6890.17065269081398113No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6870.1701573274153919No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6680.1654513751287945No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6350.1572778790520726No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC6010.1488567012760561No Hit
AATTCCCCGAACATCGAAGCGTGCGGCTATA5560.137711024807799No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA5530.1369679797099152No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5300.13127130062613934No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4960.12285012285012285No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4790.11863953396211462No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4480.11096140128398194No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4390.1087322659903305No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4360.1079892208924467No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4340.1074938574938575No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4290.10625544899738448No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4290.10625544899738448No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4240.10501704050091146No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4170.10328326860584926No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4080.10105413331219784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)