FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412683
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62271.5089063518487555No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30300.7342197279752255No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA28150.6821216284654323No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC21620.5238887960008045No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19180.4647635109757368No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19060.46185571007286463No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17460.42308503136790226No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15880.3847989861467519No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA14850.3598403617304323No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14270.3457859906998834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14260.3455436739579774No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA12890.3123462803168534No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12120.2936878911900902No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA11380.2757564522890451No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11290.273575601611891No Hit
AACTCTCCAACCGCTGAAGAGTGCGGTTACT10630.25758269664609396No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10220.24764771022794735No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9900.2398935744869549No Hit
AACTCTCCAACTGTTGAAGAATGTGGTTATA9230.2236583527792519No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9160.22196213558590977No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG8870.21493495007063532No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7820.18949169217050377No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7680.18609925778381953No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7540.18270682339713534No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7440.1802836559780752No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7050.1708333030437406No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7000.16962171933421052No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6980.1691370858503985No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6710.16259453381893607No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6690.16210990033512404No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6630.16065599988368795No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC6250.15144796369125937No Hit
AATTCCCCGAACATCGAAGCGTGCGGCTATA6130.14854016278838722No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5340.129397140177812No Hit
CTGCAGGCGCCGAAAACTCGCTCCACCACCA5340.129397140177812No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5120.1240661718558797No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5070.12285458814634961No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4660.112919601728203No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4610.11170801801867293No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4430.10734631666436466No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4430.10734631666436466No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4300.10419619901958646No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4270.1034692487938684No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4220.10225766508433834No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4210.10201534834243234No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC4130.10007681440718422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)