FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007948609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390543
Sequences flagged as poor quality0
Sequence length31
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62941.6116023075564023No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC49611.270282657735512No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG38360.9822221880817222No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36190.9266585241573911No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA29870.7648325536496622No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26750.6849437833990111No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26420.6764940096225No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22580.5781693693140064No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA22000.563318251767411No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC21740.5566608542465235No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA18330.4693465252225747No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18180.4655057189605242No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16180.41429496879985045No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC15130.38740932496549674No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14030.3592434123771262No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13270.3397833273160702No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12740.32621247852349167No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12390.31725059724537374No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC12310.3152021672389468No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12280.3144340059865367No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC11710.29983894219074464No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11590.29676629718110425No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11590.29676629718110425No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG10650.2726972446055876No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10540.26988065334675054No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9270.23736182699472275No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8470.21687752693045326No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8320.21303672066840273No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8280.21201250566518925No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8020.20535510814430166No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7790.19946587187582415No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7760.1986977106234141No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7750.19844165687261073No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7660.19613717311538037No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7540.19306452810573996No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7420.18999188309609955No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC7000.17923762556235806No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6900.17667708805432436No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6900.17667708805432436No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6310.1615699167569256No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6130.15696094924246498No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG6110.15644884174085824No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT6010.15388830423282454No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5890.15081565922318413No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5850.14979144421997065No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC5810.14876722921675717No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5710.1462066917087235No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5610.14364615420068982No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5380.1377569179322123No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5300.13570848792578538No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC5290.135452434174982No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5240.13417216542096516No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5040.1290510904048978No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5040.1290510904048978No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG4810.1231618541364203No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4710.12060131662838663No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC4580.11727261786794285No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4400.1126636503534822No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG4310.11035916659625188No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT4300.11010311284544852No Hit
ATCCACTCCCAGCGTGAACGTGACATGTTCC4090.10472598407857778No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4060.10395782282616767No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC4060.10395782282616767No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT3980.10190939281974072No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG3930.10062912406572389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)