FastQCFastQC Report
Wed 31 May 2023
EGAF00007948646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480095
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25860.5386433934950374No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA23590.4913610847853029No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT20790.4330392943063352No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC19260.40117060165175644No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15290.31847863443693436No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13390.2789031337547777No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13120.2732792468157344No Hit
TGGACGACCGCCGCCGCGACCACGACCGCGGCCACGACCGCGAC12280.2557827096720441No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11950.24890907007988003No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG11860.24703444110019893No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11390.23724471198408648No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11000.22912131973880168No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG10860.22620523021485334No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10580.22037305116695655No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG10490.21849842218727542No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8910.18558826898842937No Hit
TGGGCGGCCGCCACCACGACCGCGACCGCGGCCACGACCACGGC7940.16538393442964414No Hit
CGGGCGACCGCCACCACGACCACGACCACGACCGCGGCCACGGC7780.16205126068798884No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7760.1616346764702819No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7660.15955175538174737No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6970.1451795998708589No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6880.14330497089117777No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6850.14268009456461742No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6740.1403888813672294No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6550.13643133129901372No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6400.1333069496662119No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6340.13205719701309115No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6150.1280996469448755No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6070.12643331007404784No Hit
CAGGGTAACGGTACCACGAGAGACGTGTTTCACCTGCAGTTTCT5910.12310063633239254No Hit
AATACCGCGGCCCTGGTTGTACACGCCGTGGTGTTCATGTGCGT5900.12289234422353909No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5830.12143429946156489No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT5560.1158104125225216No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5470.11393578354284048No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5390.11226944667201283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)