FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480095
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT55941.1651860569262333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43480.905654089294827No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26830.5588477280538227No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20810.4334558785240421No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16200.3374332163425989No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14630.30473135525260625No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG14310.29806600776929565No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13190.2747372915777086No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13100.27286266259802744No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12990.27057144940063943No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12590.2622397650465012No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12530.2609900123933805No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12190.253908080692363No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11630.24224372259656946No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG11540.24036909361688832No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11150.23224570137160352No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9800.20412626667638697No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9550.19891896395505057No Hit
ATGAGCGACGAAGGTCCGGGTACCGGCCCGG8800.18329705579104136No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8160.16996636082442015No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8090.16850831606244596No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7880.1641341817765234No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7270.15142836313646257No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6990.14559618408856578No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6960.14497130776200542No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6770.14101375769378977No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6690.13934742082296211No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6650.1385142523875483No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6510.1355981628635999No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6470.1347649944281861No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6290.13101573646882386No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5950.12393380476780637No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT5950.12393380476780637No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5860.12205917578812525No Hit
ATTCCACAGGTTACCCGCAACGACACCGGCA5830.12143429946156489No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5660.11789333361105615No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG5060.10539580707984877No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA4980.10372947020902112No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA4880.10164654912048657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)