FastQCFastQC Report
Wed 31 May 2023
EGAF00007948653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514912
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA98871.9201339257970291No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC56121.089894972344789No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA55521.0782424958051084No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA51190.9941504567770802No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT50310.9770601578522156No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG40760.7915915729289664No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA33260.6459356161829595No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG31220.6063171959480456No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26180.5084363930147286No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG23680.45988440743272635No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC23060.44784351500838976No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG20150.39132900379093905No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC19240.3736560810390902No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC14690.2852914672798459No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14620.2839320116835498No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT14410.27985364489466164No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13760.267230128643341No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12670.24606146292958797No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC12280.2384873531787956No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12060.2342147784475794No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12010.23324373873593934No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11050.21459977627245044No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC10820.21013299359890622No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10290.19983997265552172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10270.1994515567708657No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10260.1992573488285377No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9890.19207165496240133No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9790.19012957553912127No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9510.184691753153937No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9500.184497545211609No Hit
GTCCACATCAAACTGCCGAAGCTGGCTGCAA9470.18391492138462495No Hit
CAGCGTCTGAAGGGTAAAGAAGGTCGTGTAC9430.1831380896153129No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9250.17964234665340872No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC8780.1705145733639923No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8450.16410571126716797No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8080.15692001740103162No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7220.1402181343608228No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG6390.12409887514759803No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6370.12371045926294201No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6170.11982630041638184No Hit
AGCGTGCACATCAAGATCCCGAAAGTTTCCC5790.11244639860791747No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5730.11128115095394941No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5500.10681436828040519No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5420.1052607047417811No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5420.1052607047417811No Hit
ATGTCCAACATGGACATCGATGGTATTAACA5310.10312441737617302No Hit
GGCGCCGAAACCGAAGAAGGTGGTGACGATC5280.10254179354918899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)