FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460048
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26760.5816784335547596No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25030.5440736618787605No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19830.43104197822835877No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA18280.3973498417556429No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15910.3458334782457483No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG15080.32779188258616493No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14880.3234445101380725No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13850.3010555420303968No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13590.29540395784787676No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG13540.29431711473585365No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC13080.2843181581052412No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12850.27931867978993496No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12080.26258129586477935No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11200.2434528570931729No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC11160.24258338260355444No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG10820.23519284944179736No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT10130.2201944144958787No Hit
CTGTGCGCGGTCGCCGCTACCCGGAGACACCAGACCGCCCGGCA9620.20910861475324313No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9470.20584808541717386No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9440.20519597954996002No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9400.20432650506034153No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8790.19106701909365978No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8480.18432859179911662No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8050.174981741035718No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7260.15780961986575315No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7000.15215803568323305No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG6810.1480280318575453No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6170.1341164400236497No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG6050.1315080165547943No Hit
ACCGCGAGACTGTGCGTTGTTCACCGCGTGGTGCGCGTCGGATT5870.12759538135151113No Hit
GGCAACGTTGGTACAAACACGAGCGCCGTTACCGCTAGAGAAGC5690.12368274614822801No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5350.11629221298647098No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5240.11390115814002018No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4960.10781483671269085No Hit
AGCAATACCTTCGTCGATCGCGCCTGCGCTCAGATCGGTTGCCA4850.10542378186624005No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4810.10455430737662157No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4740.10303272701978924No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT4610.10020693492852921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)