FastQCFastQC Report
Wed 31 May 2023
EGAF00007948720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440541
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA49731.1288393134804706No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA44241.0042198115498897No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22000.49938598223547864No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA18530.42061919321924635No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12950.29395674863406585No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC12150.27579725837095753No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG12050.273527322088069No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT11840.26876045589400305No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11490.2608156789038932No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11330.2571837808512715No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10240.23244147536778645No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9180.2083801507691679No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9060.20565622722970167No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8790.19952739926590263No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8770.19907341200932488No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8460.19203660953237042No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8180.1856807879402825No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7600.172515157499529No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA7340.16661332316401878No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6720.15253971821010984No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6660.1511777564403767No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6460.14663788387459964No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6380.1448219348482888No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT5740.13029434263780215No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5400.12257655927598113No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5390.12234956564769227No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5380.12212257201940342No Hit
CTGTTTGTGGGTGGAAACCTGGTACAGGAAAGAGCCGCTGCGAC5150.11690171856875978No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4990.11326982051613811No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4940.11213485237469385No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT4760.10804896706549448No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG4710.1069139989240502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)