FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440541
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT96432.1888995575894183No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT22040.5002939567486341No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC19820.4499013712685085No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15730.3570609772983673No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13640.30961930898599677No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12540.28465000987422284No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12470.28306105447620084No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12320.279656150051868No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12140.27557026474266866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11630.26399358969993714No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10770.2444721376670957No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10280.23334944988094183No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10180.2310795135980533No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9540.21655192138756665No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9270.21042309342376758No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8900.20202432917708No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7650.17365012564097326No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7630.17319613838439554No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7030.15957652068706432No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6940.15753357803246462No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6670.15140475006866558No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6640.150723769183799No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6020.13665016422989007No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG6020.13665016422989007No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5850.13279127254897954No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5820.132110291664113No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5630.12779741272662476No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5240.11894466122335946No Hit
GACTCCGAAGGCCACAGCGAGGACTCTGATA5150.11690171856875978No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5110.11599374405560436No Hit
ATGAAACCGCGCGCGCGTCGCCGTAAACGTG4970.1128158332595604No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4890.11099988423324957No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4880.11077289060496072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)