FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479998
Sequences flagged as poor quality0
Sequence length33
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTATT68071.418130908878787No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACTGG34860.7262530260542752No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTATC33870.7056279401164172No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCATT30300.6312526302192926No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTGGC16670.34729311372130717No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCGCG15300.31875132813053386No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCGAA14300.2979179079912833No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCGAA10110.21062587760782336No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCTAC8810.18354243142679763No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCAGC7800.1625006770861545No Hit
ACCGAACCTACTACTGATTACGGCGGTGACTCC7370.15354230642627678No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTGAG7310.15229230121792176No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTCGT7270.1514589644123517No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGGTA6940.14458393576639902No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGAGC6870.14312559635665148No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATCCG6390.13312555468981122No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGTCC5980.12458385243271847No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGAGC5890.12270884462018591No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGCAG5880.12250051041879341No Hit
AACTACCCGCGTGATTCTAAAACCATTATCAAA5590.11645881857841076No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCCGT5390.11229213455056064No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAGCC5370.1118754661477756No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACATT5320.1108337951408131No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACAAC5270.10979212413385056No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5180.10791711632131801No Hit
AAACAGACCGGTGGCGGCAAAAAGTTCTGGCTG5160.107500447918533No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAACTG5120.10666711111296298No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGATG4900.10208375868232784No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATCCA4810.10020875086979529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)