FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474497
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63441.3369947544452336No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA39640.8354109720398654No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT35350.7449994415138557No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA21530.45374364853729315No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG18340.3865145617359014No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17540.369654602663452No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15570.3281369534475455No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG12590.2653336059026717No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12340.2600648686925312No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA11520.24278341064327066No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11290.2379361724099415No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10470.22065471436068088No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9620.20274100784620344No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT9580.201898009892581No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG9320.19641852319403497No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT9070.19114978598389454No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8980.18925304058824396No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8480.17871556616796314No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8300.17492207537666202No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8050.1696533381665216No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7290.15363637704769473No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC7010.14773539137233743No Hit
GAAATGGAGAACATCCTGCAGAACCTGACTC6630.139726910812924No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6450.1359334200216229No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG6400.1348796725795948No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5970.1258174445781533No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5970.1258174445781533No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5650.11907346094917354No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5300.11169722885497695No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG5280.11127572987816572No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5260.11085423090135449No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5260.11085423090135449No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5120.10790373806367586No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC5070.10684999062164777No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4930.10389949778396913No Hit
CCACCGCGTACCCCGACTTGGGAATCTCCGG4820.10158125341150734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)