FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007948873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469749
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52871.1254946790732923No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA42510.9049513676452743No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28760.6122418568214089No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC22170.47195417127018896No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT20980.4466214936061598No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18150.3863765542875025No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17720.37722272958537434No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17150.3650885898639486No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17080.36359843235429984No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17030.36253403413312213No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG16660.3546574872964072No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15310.3259187353246095No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14720.31335883631471273No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13650.2905807143815101No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13400.2852587232756217No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG13150.2799367321697332No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA12870.27397610213113816No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12420.2643965181405389No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12400.26397075885206783No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12310.26205484205394797No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12300.2618419624097124No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12090.2573714898807661No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11870.25268813770758425No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC11800.2511979801979355No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11700.2490691837555801No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11240.23927672012074536No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC10290.21905315391836916No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC9470.20159702309105498No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9400.20010686558140622No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG9290.19776518949481534No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9160.1949977541197533No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG8690.184992410840683No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7620.16221428890748038No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7560.16093701104206715No Hit
GCAGTTTCTGTTGACTGCTCTGAATACCCGA7390.157318057090063No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7270.15476350135923653No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7170.15263470491688114No Hit
ATCGAAACCGTCGGTGACATCGTGTCCGGTC6910.14709983416675715No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6890.14667407487828607No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6720.1430551209262819No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6670.1419907227051042No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6510.1385846483973356No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6450.13730737053192235No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6450.13730737053192235No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6210.13219825907026944No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5790.12325731401237683No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5660.12048987863731483No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC5650.12027699899307927No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5590.11899972112766606No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5320.11325197073330652No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5240.1115489335794222No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5150.10963301678130237No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5150.10963301678130237No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5120.10899437784859575No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5090.10835573891588912No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5030.10707846105047589No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5020.10686558140624036No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4710.10026631243493866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)