FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479052
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29020.6057797483362974No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT23120.4826198408523501No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG21690.45276921920793567No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT21680.4525604736020307No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT19570.40851515075607653No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT18270.38137822198842714No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG17020.35528502125030265No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG16970.3542412932207777No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC16570.34589146898457784No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15600.32564314521179333No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG14970.3124921720397786No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT13250.2765879278241193No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG12480.26051451616943466No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12310.2569658408690497No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG11970.24986849026827984No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG11900.24840727102694488No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG11670.24360612209113No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11580.24172741163798503No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG11200.2337950786135952No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11020.23003765770730528No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG10470.21855664938253047No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10310.2152167196880506No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10100.21083306196404564No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG9940.20749313226956576No Hit
TCAGCATTTACCGAAGTGAGACAGGGTCAGGGTGCCGTTAGATT9730.2031094745455608No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG8990.18766229970859113No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG8980.18745355410268613No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8970.18724480849678116No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG8630.18014745789601128No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG8620.1799387122901063No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7870.1642827918472316No Hit
GCATTTACCGAAGTGGGACAGGGTCAGAGTACCGTTGCTTTCCA7600.15864666048779674No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG7530.15718544124646178No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG7220.1507143274634069No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6910.14424321368035203No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6870.14340823125673205No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6830.14257324883311207No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6730.14048579277406212No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6600.13777209989729716No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG6520.1361021350500572No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG6510.1358933894441522No Hit
CATGGTATCGCCAGAGCGCAGTACACGGGAGTTGGCGTTAGTAG6420.13401467899100725No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6220.12983976687290733No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG6150.12837854763157236No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6030.1258736003607124No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5880.12274241627213747No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5500.11481008324774762No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5480.11439259203593764No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5420.11314011840050768No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG5350.1116788991591727No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5250.10959144310012274No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5230.10917395188831275No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5000.10437280295249785No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG4960.10353782052887786No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT4830.10082412765211292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)