FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479052
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49801.0395531174068786No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA43750.9132620258343561No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28830.6018135818241026No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC23290.486168516152735No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT21900.4571528769319406No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18790.3922329934954869No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA18450.3851356428947171No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17660.3686447400282224No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17440.3640523366983125No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17360.3623823718510725No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG16760.3498576354967728No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15800.3298180573298932No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG15390.3212594874877884No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC14270.29787997962642887No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA13300.2776316558536443No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13050.2724130157060194No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13020.2717867788883044No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG12890.26907308601153945No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12410.25905329692809964No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC12250.2557133672336197No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC12210.25487838480999975No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12060.2517472007214248No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG11890.24819852542103987No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11750.24527608693836997No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11730.24485859572656No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC10760.2246102719537754No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10330.21563421089986057No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10160.21208553559947563No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG9840.20540567621051575No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC9430.19684710636841096No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9120.1903759925853561No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8480.17701627380743637No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7860.16407404624132663No Hit
GCAGTTTCTGTTGACTGCTCTGAATACCCGA7590.15843791488189174No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7340.15321927473426683No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7230.15092307306931188No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7190.1500880906456919No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7130.14883561701026193No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6960.14528694170987702No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6900.14403446807444703No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6640.13860708232091715No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6490.1354758982323422No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6410.13380593338510224No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6270.1308834949024323No Hit
ATCGAAACCGTCGGTGACATCGTGTCCGGTC6270.1308834949024323No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6250.1304660036906223No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC6060.1264998371784274No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5730.11961123218356255No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5600.1168975393067976No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5550.11585381127727262No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5350.1116788991591727No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5150.1075039870410728No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT5070.10583402219383282No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5040.10520778537611783No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5020.10479029416430785No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4880.1018678556816379No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG4870.1016591100757329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)