FastQCFastQC Report
Wed 31 May 2023
EGAF00007948911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510311
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57691.1304870951243458No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43810.8584960935586339No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26430.5179194647969572No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26300.5153719986439642No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25590.5014589142699256No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21860.4283662315725117No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19680.38564718377616786No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17780.34841498615550126No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17440.34175238237075034No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16110.31568984403628375No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA15660.3068716919682311No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15270.2992292935092522No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13470.26395668523704174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13350.2616051780188944No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11750.23025174844359614No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA11360.22260934998461723No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10800.21163564963326284No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10720.21006797815449796No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10440.20458112797882075No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10390.20360133330459268No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10270.20124982608644534No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10260.2010538671515997No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10100.19791852419406988No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9600.1881205774517892No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9390.1840054398200313No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9270.18165393260188395No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9060.17753879497012606No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9000.17636304136105238No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8980.17597112349136115No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG8870.1738155752080594No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8860.1736196162732138No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7600.14892879048266644No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7470.1463813243296735No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7400.14500961178575417No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7080.13873892587069453No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6890.13501570610862787No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6800.13325207569501735No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6770.13266419889048053No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6750.13227228102078928No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC6630.12992077380264191No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6570.12874502019356826No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA6120.1199268681255156No Hit
GATGTCCAGTCTGGTGCGATCGTTGAACCGG6070.11894707345128754No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6000.11757536090736824No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5940.11639960729829457No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT5800.11365618221045597No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5770.11306830540591913No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5760.11287234647107353No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5740.1124804286013823No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5580.10934508564385248No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5490.10758145523024196No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5430.10640570162116826No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5410.10601378375147705No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5200.10189864611971915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)