FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446293
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAGTGATTGACGTTGAAACCACTGAAG42160.9446708776521253No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC40820.9146457596242827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37980.851010434848855No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC31610.7082790901941101No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC26660.5973654079270794No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC25500.5713735147089468No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25060.5615145207296551No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG23560.52790431398207No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23510.5267839737571506No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22130.49586258354937224No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18560.4158702914901198No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC18540.415422155400152No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16730.3748658392580659No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15960.35761259979430554No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT15470.34663326559009444No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15220.3410315644654969No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA12860.2881515058492963No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12650.2834460769046344No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11750.26327995285608335No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11510.2579023197764697No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10710.23997687617775768No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10300.23079008633341774No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10210.22877347392856262No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10100.2263087254337397No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT9670.21667379949943197No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9530.21353684686965738No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT9230.20681480552014034No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9210.20636666943017254No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8980.20121310439554282No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8880.19897242394570383No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8810.1974039476308165No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8690.1947151310910097No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7850.17589341531236205No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG7550.16917137396284504No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7490.16782696569294162No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7250.16244933261332803No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA7250.16244933261332803No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6520.14609236532950326No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC6510.14586829728451936No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6490.14542016119455156No Hit
GAACCGATCTACGCTACCATCGACGACCTGG6460.14474795705959986No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC6400.14340354878969647No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6270.14049066420490575No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6200.13892218789001842No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6180.13847405180005065No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA5980.1339926909003726No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC5950.13332048676542094No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5810.13018353413564632No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5750.12883912586574292No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5750.12883912586574292No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5480.12278928865117758No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5300.11875606384146738No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5010.11225809053693427No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4750.10643232136735284No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4730.10598418527738504No Hit
TCCCACGAGGAACAGCCACGTTATGAAGACC4670.10463977700748163No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC4650.10419164091751383No Hit
CCGCCTGCGCCGCAGGCGCCGTACCAGGGCT4580.10262316460262653No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4550.10195096046767482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)