FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007948991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007948991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379938
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT54601.437076575651817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33630.8851444182998278No Hit
ATGTCCAACATGGACATCGATGGTATTAACA16680.43901899783648907No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16670.4387557969984576No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15910.41875253330806606No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13220.3479515078776011No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12640.3326858592717759No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12600.33163305591965003No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10970.2887313193205207No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10710.2818880975317025No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10560.2779400849612305No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10100.265832846411783No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9690.2550416120524928No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9450.2487247919397375No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC9400.2474087877495802No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9300.2447767793692655No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8840.23266954081981797No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8710.2292479299254089No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8300.21845669556611866No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG8000.21056067042517462No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7360.19371581679116068No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7180.18897820170659424No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6820.17950297153746136No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6630.17450215561486349No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6330.16660613047391942No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6240.1642373229316362No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6040.15897330617100686No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6000.15792050281888098No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5960.1568676994667551No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5910.15555169527659776No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5890.15502529360053482No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5830.153446088572346No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG5750.15134048186809426No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5700.15002447767793692No Hit
TTCGTGGCAAAAGGTGTAGCCGCGGACGCCA5610.1476556701356537No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5570.14660286678352785No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5420.14265485421305582No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5300.1394964441566782No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT5260.13844364080455232No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5170.1360748332622691No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5070.13344282488195444No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4640.12212518884660128No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4620.12159878717053835No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG4360.11475556538172017No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC4310.11343956119156283No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4300.11317636035353136No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4190.11028115113518522No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4070.10712274107880759No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4030.10606993772668172No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4030.10606993772668172No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC3960.10422753186046145No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG3800.10001631845195796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)