FastQCFastQC Report
Sun 21 May 2023
EGAF00007949004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416422
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC35370.8493787552050565No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT17260.4144833846434626No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA15740.3779819510016282No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT14830.35612911901868777No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14420.3462833375758245No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12690.3047389427071577No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12310.29561358429669904No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11390.2735206113029571No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG9370.22501212712104546No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8830.2120445125377622No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8630.2072416923217313No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7960.19115224459802796No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7730.18562900134959248No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG7520.18058604012276008No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6780.16281560532344594No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6720.16137475925863667No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA6490.1558515160102012No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6480.15561137499939964No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6300.15128883680497188No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT6020.1445648885025287No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5950.14288390142691787No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5620.13495924807046697No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5510.13231769695165002No Hit
ACGGCGCGGACGTTTACGACCGGCAGAGAAGATATCAGTGGTGG5430.13039656886523768No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5370.1289557228004284No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG5330.12799515875722223No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5200.12487332561680219No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5180.1243930435951991No Hit
ACCCGGGCGTTCATCGTAACGGGAAGTGTCATCGCGACCAGAGT5150.12367262056279446No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5110.1227120565195883No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG4730.11358669810912969No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA4490.10782331384989266No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT4470.10734303182828957No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG4350.10446133969867107No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4320.10374091666626643No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4290.1030204936338618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)