FastQCFastQC Report
Wed 31 May 2023
EGAF00007949073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444543
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63541.4293330453971833No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA47721.073461959810412No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25430.5720481483231094No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT22860.5142359681740574No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22690.5104118161797622No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17350.3902884535354285No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG15390.34619823054237725No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14550.3273024206882124No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC13690.30795671059942453No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11500.2586926349082091No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9940.22360041660761726No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9540.21460241191515783No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9520.21415251168053484No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8920.2006555046418457No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8860.19930580393797676No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7970.1792852434972545No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7830.17613594185489367No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7340.16511338610663084No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7070.15903973293922072No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6690.15049162848138425No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6580.1480171771909579No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6510.1464425263697775No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6510.1464425263697775No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC6460.14531777578322008No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6440.1448678755485971No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6120.13766947179462954No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6110.13744452167731805No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6050.13609482097344913No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG5960.13407026991764576No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5620.12642196592905522No Hit
CCGGCTGCCTCCCCGCCGCGTACCCCTGCCT5550.12484731510787482No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5500.12372256452131739No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5460.12282276405207145No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG5370.12079821299626808No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5150.11584931041541538No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5150.11584931041541538No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5080.11427465959423498No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC4990.1122501085384316No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG4990.1122501085384316No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG4840.10887585677875931No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC4760.10707625584026742No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4740.10662635560564444No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC4570.10280220361134919No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT4510.10145250290748027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)