FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007949094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488122
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA28300.5797730895145066No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25600.5244590491721332No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19320.39580268867209434No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17760.3638434653631674No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12910.26448305956297813No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT11030.22596809813939955No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10920.22371456316248808No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT9630.19728674388779852No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9390.1923699403018098No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA9210.18868233761231823No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG8870.18171686586550084No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8720.17864386362425788No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8510.1743416604865177No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8420.17249785914177193No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8280.16962972371661184No Hit
CAGGTCATCTTCGTCCGCATCGCCGTCTTTGTTCGGCACCTGGG7540.15446957932647984No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7300.1495527757404911No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7150.14647977349924815No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6670.13664616632727064No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC6630.13582669906293918No Hit
ACCCAGGGTGATCTGCTGAACACGATCGGAGTAGCCGCACGCTT6590.1350072317986077No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6420.13152449592519902No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA6150.1259930918909617No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT5650.11574975108681845No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5550.11370108292598981No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT5470.11206214839732691No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5400.11062808068474685No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5400.11062808068474685No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT5370.11001348023649826No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5190.10632587754700669No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5040.10325287530576373No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG4960.1016139407771008No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA4950.10140907396101793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)