FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007949115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499389
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49110.9834017168980494No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42450.8500387473492608No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG39340.7877626459533551No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT29650.5937255326008383No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT25750.5156300999821782No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25250.505617865031068No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22530.45115130689702815No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG19410.3886749608021002No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA18260.36564682041454655No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17590.3522304255800588No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16930.3390142754445933No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16390.3282010616973942No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG16100.3223939654257503No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG15570.3117809963775734No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG15120.30276998492157414No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG14140.28314600441739807No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13750.27533646115553206No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13700.274335237660421No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12390.24810318208851215No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11870.23769045773935749No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11360.22747797808922504No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC10940.21906770073029241No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10860.2174657431381148No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10680.2138613385557151No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10420.20865497638113775No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG10080.20184665661438275No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9910.1984424967310053No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9510.1904327087701171No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9470.18963172997402825No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9020.18062071851802905No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8220.16460114259625264No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7790.1559906205382978No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7110.14237398100478785No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7040.1409722681116324No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT6800.1361663953350995No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6710.13436419304389965No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC6470.12955832026736672No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6330.12675489448105584No Hit
GCGAAAACTGCGGCGGCCAAACCGGCTGCCA6310.12635440508301143No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5240.10492822228763549No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5150.10312601999643564No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5110.10232504120034683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)