FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007949144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430022
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA22150.5150899256317119No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18270.4248619838054797No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT16060.3734692643632186No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG15010.3490519089720991No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11220.26091688332224866No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG10770.2504523024403403No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10410.24208063773481356No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC9910.23045332564380427No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9450.21975619852007572No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8200.19068791829255247No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7180.1669682016268935No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6920.16092199933956866No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG6070.14115556878485286No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5990.13929519885029137No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5840.13580700522298858No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5660.13162117287022523No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5620.13069098790294448No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG4960.11534293594281224No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG4950.11511038970099205No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4870.11325001976643055No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG4590.10673872499546534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)