FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007949618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90593421
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAAAGATCGGA2070230.22851880160260205No Hit
TTTTTTTTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAAGATCGGAA1707520.18848167793553133No Hit
TTAGCAATTTAACTGTGATAAACTACCGCATTAAAAAAAAAAGATCGGAA1598550.17645321065864153No Hit
TTAGCAATTTAACTGTGATAAACTACCGCATTAGATCGGAAGAGCACACG1532540.16916680958543337No Hit
TTTTTTTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAAAGATCGGAA1508740.16653968724726712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT1351500.14918301848872667TruSeq Adapter, Index 13 (97% over 38bp)
TAGCAATTTAACTGTGATAAACTACCGCATTAAAAAAAAAAGATCGGAAG1297820.14325764339995506No Hit
TAGCAATTTAACTGTGATAAACTACCGCATTAGATCGGAAGAGCACACGT1182860.13056798020686294No Hit
TTTTTTTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAAGATCGGAAG1154420.12742867939604577No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)