FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007949619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90593421
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGCAATTTAACTGTGATAAACTACCGCATTAAAAAAAAAAAGATCGGA2157990.2382060392663613No Hit
TAGCAATTTAACTGTGATAAACTACCGCATTAAAAAAAAAAAGATCGGAA1829920.20199259281752918No Hit
TTAGCAATTTAACTGTGATAAACTACCGCATTAAAAAAAAAAGATCGGAA1639240.18094470679057367No Hit
AATGCGGTAGTTTATCACAGTTAAATTGCTAAAGATCGGAAGAGCGTCGT1551750.17128727261552468No Hit
TTTTTTTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAAAGATCGGAA1477300.16306923656189118No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1331120.14693340700755744Illumina Single End PCR Primer 1 (100% over 50bp)
TAGCAATTTAACTGTGATAAACTACCGCATTAAAAAAAAAAGATCGGAAG1272790.1404947496132197No Hit
TTTTTTTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAAGATCGGAAG1183580.13064745617675705No Hit
AATGCGGTAGTTTATCACAGTTAAATTGCTAAGATCGGAAGAGCGTCGTG1176090.1298206853232753No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)