FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007949804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17483280
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT304520.17417784305919715No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA300780.1720386563619641No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA257300.1471691810689985No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT257200.14711198356372487No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG242360.13862387378112115No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGT233610.1336190920696803No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT227800.13029591701328355No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC204690.11707757354455228No Hit
GGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCAGT201080.11501274360417495No Hit
GTTTCGAAGAAGATATAAAATATGATTAGTTCTGTGGCTGTGAATGTTAT188760.1079660109544662No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT185900.10633016230364095No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG183400.10490022467180071No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG181190.10363615980525392No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA181050.10355608329787089No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG174840.1000041182203797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)