FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007949925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007949925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18103315
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA497810.2749827863018458No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCGTATG433120.23924899942358624TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAGATCTCGTAT393780.2175181727766434TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)