FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007951203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417818
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42851.0255661555988493No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC40720.9745870211431772No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30600.7323762978138807No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA21450.5133814244479654No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT16540.39586614267456166No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16320.390600692167403No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG15580.3728896313705968No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14930.35733261850853726No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT13800.3302873499944952No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13790.3300480113350789No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13580.3250218994873366No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC13580.3250218994873366No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12800.3063534840528651No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA12480.2986946469515435No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC11380.2723673944157504No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT11000.263272525357931No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC10320.2469974965176225No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA10180.2436467552857943No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT10060.2407746913727987No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9410.22521767851073912No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8910.2132507455399241No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8830.21133603626459366No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8500.20343786050385573No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8250.19745439401844825No Hit
GAGGATAACAACAAACCGGGTAAGGAAGATG8230.19697571669961564No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8040.1924282821707059No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7850.1878808476417962No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA7670.18357275177230278No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7020.1680157389102432No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6880.16466499767841503No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6870.16442565901899872No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA6850.16394698170016608No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG6820.1632289657219172No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6800.1627502884030846No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6580.157484837895926No Hit
CTGCCGAAACTGGCAGCAAACCGTGAGAAAC6560.15700616057709338No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6480.15509145130176297No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6420.15365541934526517No Hit
ACCAAAGCCGCTATCAGCCAGCTGGAAAAAC6340.15174071006993475No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6150.14719327554102504No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6080.14551790492511094No Hit
AAATACAACCTGCAGGTCCGCGGCACCCGTG5970.1428851796715316No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5940.14216716369328272No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5880.14073113173678492No Hit
ATCGATGAAGACAACCCGACCGATAACGGCA5880.14073113173678492No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5740.1373803905049567No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5620.1345083265919611No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5560.13307229463546327No Hit
GGTAAAGAAGACAACAAAAAACCGGGCAAAG5520.13211493999779808No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5480.1311575853601329No Hit
AAGTTCAACCTGCAGGTTCGTGGTACCCGCG5390.12900353742538617No Hit
AAATACCACCTGCAGGTTCGTGGCACCCGTG5180.12397742557764385No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5170.12373808691822756No Hit
AACGACGGTCAGGAAAGCGAGCAGAACACCT5160.12349874825881126No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC5150.12325940959939496No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5140.12302007093997866No Hit
TACAACCTGCAGGTGCGCGGCACTGCCGGTG5030.12038734568639935No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT4890.11703660445457112No Hit
AACCAGAAAGTCGACGTTCTGACCAAAGCAG4880.11679726579515483No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG4870.11655792713573852No Hit
CTGCAGGTCCGCGGCACCCGTGGTGAACACA4740.11344652456332661No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG4680.1120104926068288No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG4660.1115318152879962No Hit
CGTTACAACCTGCAGGTCCGTGGCACTGCCG4630.11081379930974732No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC4600.11009578333149841No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG4490.1074630580779191No Hit
AAACTGGAGGAAGTTGCCGGTAAATACTCCC4450.10650570344025388No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA4440.10626636478083759No Hit
GCCGAAGTGCTGGCCCAAGTTGTTAAATCTC4430.10602702612142129No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4370.10459099416492347No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4320.10339430086784199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)