FastQCFastQC Report
Wed 31 May 2023
EGAF00007951223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413302
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT70291.7006934396639746No Hit
ATGTCCAACATGGACATCGATGGTATTAACA17400.42099965642556775No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC15060.36438246125109486No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13500.3266376644681129No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13180.318895142051091No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12410.30026469748513196No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC11990.29010263681279064No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10490.25380956298300034No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10400.2516319785532129No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9340.22598487304682774No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC8580.20759638230640065No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8430.20396707492342162No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7840.1896917992170374No Hit
GGCCGCTCCGACGCGTCCCATGGCCAGTCTG7190.1739648005574616No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6440.15581826364256646No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6280.15194700243405548No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA6070.14686597209788485No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT6030.1458981567957571No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5900.14275275706384194No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5530.13380046551916033No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5350.12944529665958548No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5100.1233964510212871No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG4930.11928323598724419No Hit
TCTGGCTACGAAATTGACCACATCGACCTGG4710.11396025182554162No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA4710.11396025182554162No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4710.11396025182554162No Hit
GGTCAGAAAGGTGGCGCGCAGAAGTCCCAGA4690.11347634417447774No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4640.11226657504681806No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4550.11008899061703065No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA4520.10936312914043485No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT4340.10500796028086001No Hit
TCCTGGGCCCCGAGCGTATGCGCGCTGTCTG4260.10307232967660453No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4150.10041083759575323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)