FastQCFastQC Report
Wed 31 May 2023
EGAF00007951224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399215
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC34810.8719612239019076No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT31850.7978157133374247No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA29430.7371967486191652No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA24920.6242250416442269No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG24870.6229725836955025No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA21900.5485765815412748No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG19460.4874566336435254No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG18360.4599025587715892No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG18260.4573976428741405No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18190.4556442017459264No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG17590.44061470636123395No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT16210.40604686697644127No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG14370.3599564144633844No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT14270.35745149856593567No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14100.35319314154027276No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13510.3384141377453252No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13320.3336547975401726No Hit
ATCGCTAATCTGTACTTTTTTAGCTTCGCGGCCACGAGAACGGG12490.31286399559134803No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC12300.3081046553861954No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG12170.30484826471951204No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG12020.30109089087333896No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG11870.2973335170271658No Hit
GATCGCCGGGGACGGAGTTTCCGGACGAGCAGGGGATTCCCAGG10770.26977944215522964No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG10440.2615132196936488No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA10300.25800633743722057No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10080.2524955224628333No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9910.24823716543717045No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA9110.2281978382575805No Hit
AACAGCGGTAGACGGAGCGGAAGAGGCCAGAGCGCCAGTACCCG8790.22018210738574454No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8600.2154227671805919No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7970.19964179702666482No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7630.1911250829753391No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG7600.1903736082061045No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7530.18862016707789037No Hit
AATCAGCGGGTTGAATGCCTGTTTTACCTTTTTCGGGTGTTTCT7270.18210738574452362No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7230.18110541938554414No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6570.16457297446238242No Hit
GATTGCCGGGGATGGAGTTTCCGGGCGCGCCGGGGACTCCCAGG6330.15856117630850544No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6320.15831068471876056No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6210.15555527723156695No Hit
TACCTGGATGTCCAGATCGAAGTAGAAACGGCGTGCCATGGCCG6190.15505429405207719No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6030.1510464286161592No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5910.14804052953922073No Hit
GATGAGCGGGTTGAACGCCTGTTTAACCTTTTTAGGGTGTTTCT5820.14578610523151683No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5810.14553561364177198No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5730.143531680923813No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5700.14278020615457837No Hit
ATCGCTAGTCACAGTGATTTTCAGTTTACGGCCTGCGGAGGTGG5690.1425297145648335No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA5660.14177823979559886No Hit
GAAAACCAGGATACGGTCCGCTTTCGGCGCTTCTTTCGCAGCTG5580.13977430707763988No Hit
TTTGGAGGTGAACGGTTTACCTTTATCCGGCAGGTCTGCCATCG5550.13902283230840523No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT5440.13626742482121162No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5370.13451398369299752No Hit
CGGACGCTCCAGTGCGGTGTTGCTAGAGTGGCTCGGGATCGCCG5360.13426349210325264No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5330.13351201733401802No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5250.131508084616059No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5170.12950415189810002No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG4800.12023596307753966No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC4790.1199854714877948No Hit
TTTCGGGTGTTTCTGCTTCTGACGGGCTTCGCTGGAGGTACGTT4730.11848252194932554No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA4480.1122202322057037No Hit
GGCAATGCCGGCGCGCCAACGACCATCACGCGCTTTGTCAGTGA4260.10670941723131645No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4250.10645892564157158No Hit
GATCAGCGGGTTGAATGCCTGTTTAACTTTCTTCGGGTGTTTCT4240.1062084340518267No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG4130.1034530265646331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)