FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007951225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399215
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC47051.1785629297496336No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT31670.793306864722017No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA29330.7346918327217165No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT28890.7236702027729419No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27580.6908558045163634No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26690.6685620530290696No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21670.5428152749771427No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20450.512255301028268No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG19640.49196548225893316No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19110.47868942800245484No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG18350.45965206718184437No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG18300.45839960923312No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18210.4561451849254161No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18030.4516363363100084No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG15170.37999574164297434No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14880.372731485540373No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14440.3617098555915985No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13810.3459288854376714No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT13620.34116954523251886No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13360.33465676389915205No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT12860.3221321844119084No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12830.3213807096426738No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA12210.3058502310784915No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12010.300840399283594No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11820.29608105907844146No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG10990.27529025712961686No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10990.27529025712961686No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10300.25800633743722057No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9980.24999060656538458No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC9940.24898864020640507No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9740.2439788084115076No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9240.23145422892426387No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8770.2196811242062548No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8200.20540310359079694No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8020.20089425497538918No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7670.1921270493343186No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7640.19137557456508397No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7570.18962213343686984No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7360.1843618100522275No Hit
GCCCGCCCGGAAACTCCATCCCCGGCAATCC7040.1763460791803915No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG6860.1718372305649838No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6690.1675788735393209No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC6640.16632641559059655No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6600.16532444923161707No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6530.16357100810340294No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC6310.1580601931290157No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6190.15505429405207719No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6140.15380183610335282No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6030.1510464286161592No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5980.14979397066743483No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5920.14829102112896558No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5810.14553561364177198No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5720.1432811893340681No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5640.14127725661610913No Hit
GACGGTACCTCTAACGCCCTGTACCGTACCA5540.13877234071866038No Hit
CTGGATGCTCACGCAATTAAGCCGGCCATCG5510.13802086594942575No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5480.13726939118019113No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5450.13651791641095648No Hit
GCTTCTGAACCGGAAGACAAATCTCCGCGTG4860.12173891261600893No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4740.11873301353907043No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC4610.11547662287238705No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG4560.11422416492366269No Hit
CCACCGCGTACCCCGACTTGGGAATCTCCGG4400.1102162994877447No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG4350.10896384153902033No Hit
CCGCCTCGTACCCCAACCTGGGAATCTCCGG4310.10796187518004083No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4270.10695990882106134No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4180.10470548451335746No Hit
GCACGCCCGGAAACCCCGTCCCCGGCAATTC4080.1022005686159087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)