FastQCFastQC Report
Wed 31 May 2023
EGAF00007951227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390162
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57811.481692220154705No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT50981.3066367303837894No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG48001.2302582004398173No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT37810.9690846366381144No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC28650.734310363387516No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26340.6751041874913498No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG26290.6738226685325582No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24250.621536695013866No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23960.6141038850528755No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA21730.5569481394907756No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG15800.4049599909781066No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14740.3777917890517272No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14560.3731783208000779No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13430.34421599233139055No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12000.3075645501099543No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11700.2998754363572055No Hit
GCTCGTCAGCGCTACCAATCCGCATCTCCGG10620.27219462684730955No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9530.24425751354565536No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8820.22605994433081644No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8670.222215387454442No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8150.20888759028301063No Hit
ATGTCCAACATGGACATCGATGGTATTAACA8140.20863128649125234No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7690.19709761586212907No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7630.19555979311157928No Hit
CAGCCGCAGCGCCGTAAACGTCGTGCGGCTC6570.1683915911852No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6420.16454703430882558No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6360.16300921155827577No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6220.15942095847365967No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6160.15788313572310989No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5920.1517318447209108No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5910.15147554092915252No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5890.1509629333456359No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5790.14839989542805296No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5780.14814359163629465No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA5700.1460931613022283No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5590.14327381959288707No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5500.1409670854670624No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5390.13814774375772115No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5330.1366099210071714No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5150.13199645275552208No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG4820.12353842762749831No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4710.12071908591815707No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4560.11687452904178264No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4330.11097954183134186No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG4100.10508455462090106No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC3970.10175260532804323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)