FastQCFastQC Report
Wed 31 May 2023
EGAF00007951232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007951232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444103
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA35760.8052186091965152No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA30740.6921817686437606No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT30030.6761944864141877No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC24760.5575283211327102No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC23420.5273551405867558No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT22230.500559554878035No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19240.4332328311225099No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16680.375588545900388No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16120.3629788585080488No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT16050.3614026475840064No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA15580.35081951709400744No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15500.34901813318081615No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13230.2979038646440128No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC13160.29632765371997033No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11780.26525378121742027No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10710.2411602713784865No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG10200.22967644893189193No Hit
CGCGCCGAAGTTAGATTTATTTTCGGTACCGTCCAGATCGATCA9530.21458985865891472No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG9520.2143646856697658No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8950.20152982528827773No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8930.20107947930997988No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG8470.19072152180912985No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA7770.1749594125687059No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7520.16933008783998307No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7090.15964764930657976No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7020.15807143838253737No Hit
AACGTGACCTTCCTCATCTTTACGCCAGTGCTGCGCCTCATCGA6990.15739591941509065No Hit
ACCCAGGTCAGTACCGTTGGTGGTGCCGGTCAGCGCAGTGGTGC6600.14861417283828302No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6540.14726313490338955No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6520.1468127889250917No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG6450.1452365780010493No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6130.13803104234828406No Hit
ACCCTGGTGCAGACCCAGGACTGGAGACAGTTCCGGCATGGACG6110.13758069636998624No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5990.13487862050019928No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5460.12294445207530685No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4780.10763268881318074No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT4760.1071823428348829No Hit
CGGTTCATGGATCGGGCTGACGTTAGGGGTAGCAGTGTGGGAGT4750.10695716984573397No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT4680.10538095892169158No Hit
GATCGCCGGGGACGGAGTTTCCGGACGAGCAGGGGATTCCCAGG4530.10200336408445788No Hit
GATTGCCGGAGATGGAGTTTCCGGACGCGCCGGAGATTCCCAGG4500.10132784511701115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)